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For in vitro experiment, 5000 cells were seeded in a 96-well white plate and incubated in normal culture conditions for 24 h

Pharmacological agents, such as corticosteroids, PPAR agonists, and NF-B inhibitors, have been investigated for their ability to modulate macrophage function and polarization in COPD (115)

Co-immunoprecipitation The recombinant GST-tagged Nanobody against GFP 69 expressed in Escherichia coli Rosettagami (Novagen, 71351) was purified with Glutathione Sepharose 4B beads according to the manufacturers instructions

As multi-omics technologies advance, extensive genomic data, even at the single-cell level, is increasingly accessible (Atherine et al., 2022)

Puerarin improves the bone micro-environment to inhibit OVX-induced osteoporosis via modulating SCFAs released by the gut microbiota and repairing intestinal mucosal integrity